In Vivo Model of Osteoarthritis to Compare Allogenic Amniotic Epithelial Stem Cells and Autologous Adipose Derived Cells.
Francesca VeronesiMilena FiniLucia MartiniPaolo BerardinelliValentina RussoGiuseppe FilardoBerardo Di MatteoMaurilio MarcacciElizaveta KonPublished in: Biology (2022)
The challenge of osteoarthritis (OA) is to find a minimally invasive orthobiological therapy to contrast OA progression, on inflammatory and structural fronts. The aim of the present study is to compare the effects of an intra-articular injection of three orthobiological treatments, autologous culture expanded adipose-derived mesenchymal stromal cells (ADSCs), autologous stromal vascular fraction (SVF) and allogenic culture expanded amniotic epithelial stem cells (AECs), in an animal model of OA. OA was induced in 24 sheep by bilateral lateral meniscectomy and, at 3 and 6 months post-treatment, the results were analyzed with macroscopy, histology, histomorphometry, and biochemistry. All the three treatments showed better results than control (injection of NaCl), but SVF and AECs showed superiority over ADSCs, because they induced higher cartilage regeneration and lower inflammation. SVF showed better results than AECs at 3 and 6 months. To conclude, SVF seems to be more favorable than the other biological options, because it is easily obtained and rapidly used after harvesting, with good healing potential. AECs cause no discomfort and could be also considered for the treatment of OA joints.
Keyphrases
- stem cells
- knee osteoarthritis
- bone marrow
- cell therapy
- minimally invasive
- oxidative stress
- high glucose
- diabetic rats
- rheumatoid arthritis
- induced apoptosis
- magnetic resonance
- platelet rich plasma
- ultrasound guided
- umbilical cord
- mesenchymal stem cells
- computed tomography
- magnetic resonance imaging
- endothelial cells
- combination therapy
- risk assessment
- cell death
- cell cycle arrest
- cell proliferation
- energy transfer