Oxidised Albumin Levels in Plasma and Skeletal Muscle as Biomarkers of Disease Progression and Treatment Efficacy in Dystrophic mdx Mice.
Jessica R TerrillAngelo Patrick R BautistaIrene TsioutsiasMiranda D GroundsPeter G ArthurPublished in: Antioxidants (Basel, Switzerland) (2024)
Redox modifications to the plasma protein albumin have the potential to be used as biomarkers of disease progression and treatment efficacy in pathologies associated with inflammation and oxidative stress. One such pathology is Duchenne muscular dystrophy (DMD), a fatal childhood disease characterised by severe muscle wasting. We have previously shown in the mdx mouse model of DMD that plasma albumin thiol oxidation is increased; therefore, the first aim of this paper was to establish that albumin thiol oxidation in plasma reflects levels within mdx muscle tissue. We therefore developed a method to measure tissue albumin thiol oxidation. We show that albumin thiol oxidation was increased in both mdx muscle and plasma, with levels correlated with measures of dystropathology. In dystrophic muscle, albumin content was associated with areas of myonecrosis. The second aim was to test the ability of plasma thiol oxidation to track acute changes in dystropathology: we therefore subjected mdx mice to a single treadmill exercise session (known to increase myonecrosis) and took serial blood samples. This acute exercise caused a transient increase in total plasma albumin oxidation and measures of dystropathology. Together, these data support the use of plasma albumin thiol oxidation as a biomarker to track active myonecrosis in DMD.
Keyphrases
- duchenne muscular dystrophy
- skeletal muscle
- hydrogen peroxide
- oxidative stress
- muscular dystrophy
- mouse model
- electron transfer
- early onset
- small molecule
- intensive care unit
- hepatitis b virus
- body composition
- blood brain barrier
- climate change
- endoplasmic reticulum stress
- deep learning
- respiratory failure
- binding protein
- subarachnoid hemorrhage
- data analysis