Chaetolines A and B, Pyrano[3,2- f]isoquinoline Alkaloids from Cultivation of Chaetomium sp. in the Presence of Autoclaved Pseudomonas aeruginosa.
Elena AncheevaAttila MándiSándor B KirályTibor KurtánRudolf HartmannSergi H AkoneHorst WeberGeorgios DaletosPeter ProkschPublished in: Journal of natural products (2018)
The first members of a new alkaloid class, chaetolines A (1) and B (2), which feature a pyrano[3,2- f]isoquinoline core structure, were obtained from a crude extract of the fungal endophyte Chaetomium sp. after cultivation in the presence of autoclaved Pseudomonas aeruginosa. The structures of the new compounds, including the absolute configuration of the major stereoisomer, were determined through detailed analysis of HRESIMS, 1D/2D NMR, and calculation of ECD data. The possible biosynthetic origin of the unprecedented scaffold of 1 and 2 is proposed. The current study provides further evidence for mixed fermentation as a powerful tool to induce the accumulation of cryptic fungal natural products even in the absence of viable bacterial cells.
Keyphrases
- pseudomonas aeruginosa
- cystic fibrosis
- induced apoptosis
- biofilm formation
- high resolution
- acinetobacter baumannii
- oxidative stress
- magnetic resonance
- cell cycle arrest
- electronic health record
- deep learning
- endoplasmic reticulum stress
- escherichia coli
- cell death
- drug resistant
- multidrug resistant
- candida albicans