Targeted Redox Balancing through Pulmonary Nanomedicine Delivery Reverses Oxidative Stress Induced Lung Inflammation.
Ria GhoshMonojit DasSusmita MondalAmrita BanerjeeLopamudra RoyAnjan Kumar DasSamir Kumar PalSiddhartha Sankar BhattacharyaMaitree BhattacharyyaSamir Kumar PalPublished in: ChemMedChem (2024)
Non-invasive delivery of drugs is important for the reversal of respiratory diseases essentially by-passing metabolic pathways and targeting large surface area of drug absorption. Here, we study the inhalation of a redox nano medicine namely citrate functionalized Mn 3 O 4 (C-Mn 3 O 4 ) duly encapsulated in droplet evaporated aerosols for the balancing of oxidative stress generated by the exposure of Chromium (VI) ion, a potential lung carcinogenic agent. Our optical spectroscopic in-vitro experiments demonstrates the efficacy of redox balancing of the encapsulated nanoparticles (NP) for the maintenance of a homeostatic condition. The formation of Cr-NP complex as an excretion of the heavy metal is also demonstrated through optical spectroscopic and high resolution transmission optical microscopy (HRTEM). Our studies confirm the oxidative stress mitigation activity of the Cr-NP complex. A detailed immunological assay followed by histopathological studies and assessment of mitochondrial parameters in pre-clinical mice model with chromium (Cr) induced lung inflammation establishes the mechanism of drug action to be redox-buffering. Thus, localised delivery of C-Mn 3 O 4 NPs in the respiratory tract via aerosols can act as an effective nanotherapeutic agent against oxidative stress induced lung inflammation.
Keyphrases
- oxidative stress
- high resolution
- diabetic rats
- respiratory tract
- high speed
- ischemia reperfusion injury
- induced apoptosis
- heavy metals
- dna damage
- high throughput
- cancer therapy
- molecular docking
- mass spectrometry
- drug induced
- single molecule
- risk assessment
- climate change
- emergency department
- metal organic framework
- electron transfer
- type diabetes
- adverse drug
- endothelial cells
- optical coherence tomography
- skeletal muscle
- human health
- heat shock protein
- sewage sludge