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The buffy coat method: a tool for detection of blood parasites without staining procedures.

Carolina Romeiro Fernandes ChagasRasa BinkienėMikas IlgūnasTatjana IezhovaGediminas Valkiūnas
Published in: Parasites & vectors (2020)
BCM is sensitive and recommended as a quick and reliable tool to detect Haemoproteus, Trypanosoma and microfilariae parasites during fieldwork. However, it is not suitable for detection of species of Leucocytozoon and Plasmodium. BCM is a useful tool for diagnostics of blood parasite co-infections. Its application might be extended to studies of blood parasites in other vertebrates during field studies.
Keyphrases
  • plasmodium falciparum
  • loop mediated isothermal amplification
  • real time pcr
  • case control
  • label free
  • flow cytometry