The genetic source tracking of human urinary exosomes.
Qingfu ZhuLiming ChengChunyu DengLiu HuangJiaoyuan LiYong WangMeng LiQinsi YangXianjun DongJianzhong SuLuke P LeeLuke P LeePublished in: Proceedings of the National Academy of Sciences of the United States of America (2021)
The genetic origins of nanoscale extracellular vesicles in our body fluids remains unclear. Here, we perform a tracking analysis of urinary exosomes via RNA sequencing, revealing that urine exosomes mostly express tissue-specific genes for the bladder and have close cell-genetic relationships to the endothelial cell, basal cell, monocyte, and dendritic cell. Tracking the differentially expressed genes of cancers and corresponding enrichment analysis show urine exosomes are intensively involved in immune activities, indicating that they may be harnessed as reliable biomarkers of noninvasive liquid biopsy in cancer genomic diagnostics and precision medicine.
Keyphrases
- genome wide
- mesenchymal stem cells
- endothelial cells
- single cell
- dendritic cells
- stem cells
- copy number
- cell therapy
- dna methylation
- papillary thyroid
- spinal cord injury
- squamous cell carcinoma
- gene expression
- ionic liquid
- regulatory t cells
- bone marrow
- vascular endothelial growth factor
- genome wide identification
- transcription factor
- atomic force microscopy
- squamous cell
- induced pluripotent stem cells
- lymph node metastasis
- single molecule