Silk fibroin hydrogel adhesive enables sealed-tight reconstruction of meniscus tears.
Xihao PanRui LiWenyue LiWei SunYiyang YanXiaochen XiangJing-Hua FangYouguo LiaoChang XieXiaozhao WangYouzhi CaiXudong YaoChun-Hui YuanPublished in: Nature communications (2024)
Despite orientationally variant tears of the meniscus, suture repair is the current clinical gold treatment. However, inaccessible tears in company with re-tears susceptibility remain unresolved. To extend meniscal repair tools from the perspective of adhesion and regeneration, we design a dual functional biologic-released bioadhesive (S-PIL10) comprised of methacrylated silk fibroin crosslinked with phenylboronic acid-ionic liquid loading with growth factor TGF-β1, which integrates chemo-mechanical restoration with inner meniscal regeneration. Supramolecular interactions of β-sheets and hydrogen bonds richened by phenylboronic acid-ionic liquid (PIL) result in enhanced wet adhesion, swelling resistance, and anti-fatigue capabilities, compared to neat silk fibroin gel. Besides, elimination of reactive oxygen species (ROS) by S-PIL10 further fortifies localized meniscus tear repair by affecting inflammatory microenvironment with dynamic borate ester bonds, and S-PIL10 continuously releases TGF-β1 for cell recruitment and bridging of defect edge. In vivo rabbit models functionally evidence the seamless and dense reconstruction of torn meniscus, verifying that the concept of meniscus adhesive is feasible and providing a promising revolutionary strategy for preclinical research to repair meniscus tears.
Keyphrases
- ionic liquid
- anterior cruciate ligament
- anterior cruciate ligament reconstruction
- tissue engineering
- rotator cuff
- wound healing
- growth factor
- stem cells
- reactive oxygen species
- room temperature
- transforming growth factor
- rheumatoid arthritis
- combination therapy
- oxidative stress
- single cell
- squamous cell carcinoma
- cell death
- drug delivery
- dna damage
- epithelial mesenchymal transition
- photodynamic therapy
- depressive symptoms
- cancer therapy
- staphylococcus aureus
- cystic fibrosis
- escherichia coli
- pseudomonas aeruginosa
- quantum dots
- bone regeneration
- cell migration
- silver nanoparticles
- locally advanced
- mesenchymal stem cells