Ramanomics: New Omics Disciplines Using Micro Raman Spectrometry with Biomolecular Component Analysis for Molecular Profiling of Biological Structures.
Andrey N KuzminArtem PlissParas N PrasadPublished in: Biosensors (2017)
Modern instrumentation for Raman microspectroscopy and current techniques in analysis of spectral data provide new opportunities to study molecular interactions and dynamics at subcellular levels in biological systems. Implementation of biomolecular component analysis (BCA) to microRaman spectrometry provides basis for the emergence of Ramanomics, a new biosensing discipline with unprecedented capabilities to measure concentrations of distinct biomolecular groups in live cells and organelles. Here we review the combined use of microRaman-BCA techniques to probe absolute concentrations of proteins, DNA, RNA and lipids in single organelles of live cells. Assessing biomolecular concentration profiles of organelles at the single cell level provides a physiologically relevant set of biomarkers for cellular heterogeneity. In addition, changes to an organelle's biomolecular concentration profile during a cellular transformation, whether natural, drug induced or disease manifested, can provide molecular insight into the nature of the cellular process.
Keyphrases
- single cell
- drug induced
- induced apoptosis
- liver injury
- cell cycle arrest
- high resolution
- single molecule
- rna seq
- healthcare
- primary care
- high throughput
- cell death
- endoplasmic reticulum stress
- oxidative stress
- signaling pathway
- magnetic resonance imaging
- deep learning
- nucleic acid
- liquid chromatography
- raman spectroscopy
- data analysis