Facile Preparation of Fe3O4/C Nanocomposite and Its Application for Cost-Effective and Sensitive Detection of Tryptophan.
Jun LiuShuai DongQuanguo HeSuchun YangMei XiePeihong DengYonghui XiaGuangli LiPublished in: Biomolecules (2019)
In this study, we reported facile synthesis of Fe3O4/C composite and its application for the cost-effective and sensitive determination of tryptophan (Trp) in human serum samples. Fe3O4/C composites were prepared by a simple one-pot hydrothermal method followed by a mild calcination procedure, using FeCl3∙6H2O as Fe3O4 precursor, and glucose as reducing agent and carbon source simultaneously. The Fe3O4/C composite modified glassy carbon electrode (Fe3O4/C/GCE) was prepared by drop-casting method. The microstructure and morphology of Fe3O4/C composite was characterized by powder X-ray diffraction (XRD) and scanning electron microscopy (SEM), respectively. Due to large specific surface area and synergistic effect from Fe3O4 nanoparticles and carbon coating, Fe3O4/C composite showed excellent electrocatalytic activity toward the oxidation of Trp. As a result, the proposed Fe3O4/C/GCE displayed superior analytical performances toward Trp determination, with two wide detection ranges (1.0-80 μM and 80-800 μM) and a low detection limit (0.26 μM, S/N = 3). Moreover, successful detection of Trp in human serum samples further validate the practicability of the proposed sensor.
Keyphrases
- electron microscopy
- loop mediated isothermal amplification
- sensitive detection
- reduced graphene oxide
- quantum dots
- solid phase extraction
- molecularly imprinted
- real time pcr
- label free
- high resolution
- type diabetes
- white matter
- mass spectrometry
- gold nanoparticles
- highly efficient
- nitric oxide
- magnetic resonance
- minimally invasive
- computed tomography
- walled carbon nanotubes
- metal organic framework
- solid state