Optimizing depth and type of high-throughput sequencing data for microsatellite discovery.
Mark A ChapmanPublished in: Applications in plant sciences (2019)
Small high-throughput sequencing resources may be sufficient for identification of hundreds of SSRs. Genomic data may be preferable in species with low polymorphism, but transcriptome data may result in longer loci (more amenable to primer design) and primers may be more transferable to related species.