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Downsizing Class II Lasso Peptides: Genome Mining-Guided Isolation of Huascopeptin Containing the First Gly1-Asp7 Macrocycle.

Carlos Cortés-AlbayayScott Alexander JarmuschFranziska TruschRainer EbelBarbara A AndrewsMarcel JasparsJuan A Asenjo
Published in: The Journal of organic chemistry (2020)
A new lasso peptide, huascopeptin, was isolated following genome-mined discovery of a new biosynthetic gene cluster in extremotolerant Streptomyces huasconensis HST28T from Salar de Huasco, Atacama Desert, Chile. Compound 1 is a 13-residue class II lasso peptide containing a novel Gly1-Asp7 macrolactam ring, a three-residue loop, and a three-residue tail, making it the smallest lasso peptide isolated to date. The lasso structure was confirmed using NOE restraint-based molecular dynamics simulations.
Keyphrases
  • molecular dynamics simulations
  • genome wide
  • amino acid
  • small molecule
  • molecular docking
  • high throughput
  • single cell
  • genome wide identification
  • genome wide analysis