Light-field microscopy with temporal focusing multiphoton illumination for scanless volumetric bioimaging.
Feng-Chun HsuChun-Yu LinYvonne Yuling HuYeu-Kuang HwuAnn-Shyn ChiangShean-Jen ChenPublished in: Biomedical optics express (2022)
A temporal focusing multiphoton illumination (TFMI) method is proposed for achieving selective volume illumination (SVI) (i.e., illuminating only the volume of interest) in light-field microscopy (LFM). The proposed method minimizes the background noise of the LFM images and enhances the contrast, and thus improves the imaging quality. Three-dimensional (3D) volumetric imaging is achieved by reconstructing the LFM images using a phase-space deconvolution algorithm. The experimental results obtained using 100-nm fluorescent beads show that the proposed TFMI-LFM system achieves lateral and axial resolutions of 1.2 µm and 1.1 µm, respectively, at the focal plane. Furthermore, the TFMI-LFM system enables 3D images of the single lobe of the drosophila mushroom body with GFP biomarker (OK-107) to be reconstructed in a one-snapshot record.
Keyphrases
- high resolution
- deep learning
- optical coherence tomography
- convolutional neural network
- single molecule
- quantum dots
- living cells
- label free
- high throughput
- machine learning
- high speed
- magnetic resonance
- mass spectrometry
- photodynamic therapy
- minimally invasive
- computed tomography
- fluorescence imaging
- single cell
- quality improvement