Advances in RT-LAMP for COVID-19 testing and diagnosis.

Gihoon ChoiTaylor J Moehling Robert J Meagher

Published in: Expert review of molecular diagnostics (2023)

RT-LAMP occupies a middle ground between RT-qPCR and rapid antigen tests. The simplicity approaches that of rapid antigen tests, making it suitable for point-of-care use, but the sensitivity nears that of RT-qPCR. RT-LAMP still lags RT-qPCR in fundamental understanding of the mechanism, and the interplay between sample preparation and assay performance. Industry is now beginning to address issues around scalability and usability, which could finally enable LAMP and RT-LAMP to find future widespread application as a diagnostic for other conditions, including other pathogens with pandemic potential.

Keyphrases

loop mediated isothermal amplification
coronavirus disease
sars cov
sensitive detection
high throughput
health information
climate change
social media
gram negative
mass spectrometry
current status
multidrug resistant
respiratory syndrome coronavirus
tandem mass spectrometry