High-definition spatial transcriptomics for in situ tissue profiling.
Sanja VickovicGökçen EraslanFredrik SalménJohanna KlughammerLinnea StenbeckDenis SchapiroTarmo ÄijöRichard BonneauLudvig BergenstråhleJosé Fernandéz NavarroJoshua GouldGabriel K GriffinÅke BorgMostafa RonaghiJonas FrisénJoakim LundebergAviv RegevPatrik L StåhlPublished in: Nature methods (2019)
Spatial and molecular characteristics determine tissue function, yet high-resolution methods to capture both concurrently are lacking. Here, we developed high-definition spatial transcriptomics, which captures RNA from histological tissue sections on a dense, spatially barcoded bead array. Each experiment recovers several hundred thousand transcript-coupled spatial barcodes at 2-μm resolution, as demonstrated in mouse brain and primary breast cancer. This opens the way to high-resolution spatial analysis of cells and tissues.