A sensitive and simple RT-LAMP assay for sarbecovirus screening in bats.
Tony Tat-Yin ChanFranklin Wang-Ngai ChowJoshua FungFlora Ka-Kei ChengGeorge Chi-Shing LoChi-Ching TsangHayes Kam-Hei LukAntonio Cheuk-Pui WongZirong HeKam Leng Aw-YongXueyan LiuKwok-Yung YuenPatrick Chiu-Yat WooSusanna Kar-Pui LauPublished in: Microbiology spectrum (2023)
We report the application of a colorimetric and fluorescent reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay to facilitate mass screening for sarbecoviruses in bats. The assay was evaluated using a total of 838 oral and alimentary samples from bats and demonstrated comparable sensitivity and specificity to quantitative reverse transcription PCR (qRT-PCR), with a simple setup. The addition of SYTO9, a fluorescent nucleic acid stain, also allows for quantitative analysis. The scalability and simplicity of the assay are believed to contribute to improving preparedness for detecting emerging coronaviruses by applying it to field studies and surveillance.