Fecal Filobasidium Is Associated with Clinical Remission and Endoscopic Response following Fecal Microbiota Transplantation in Mild-to-Moderate Ulcerative Colitis.
Isabelle A M van ThielShafaque RahmanTheodorus B M HakvoortMark DavidsCaroline VerseijdenPatricia H P van HamersveldMèlanie V BénardMaarten H LoddersTeun BoekhoutRené M van den WijngaardSigrid E M HeinsbroekCyriel Y PonsioenWouter J de JongePublished in: Microorganisms (2022)
Fecal microbiota transplantation (FMT) has the potential to restore (bacterial and fungal) microbial imbalance in ulcerative colitis (UC) patients and contribute to disease remission. Here, we aimed to identify fecal fungal species associated with the induction of clinical remission and endoscopic response to FMT for patients with mild-to-moderate ulcerative colitis. We analyzed the internal transcribed spacer 1 (ITS1)-based mycobiota composition in fecal samples from patients ( n = 31) and donors ( n = 7) that participated previously in a double-blinded randomized control trial evaluating the efficacy of two infusions of donor FMT compared with autologous FMT. The abundance of the yeast genus Filobasidium in fecal material used for transplantation was shown to correlate with clinical remission following FMT, irrespective of its presence in the material of donor or autologous fecal microbiota transfer. The amplified sequence variants within the genus Filobasidium most closely resembled Filobasidium magnum . Monocyte-derived macrophages and HT29 epithelial cells were stimulated with fungal species. Especially Filobasidium floriforme elicited an IL10 response in monocyte-derived macrophages, along with secretion of other cytokines following stimulation with other Filobasidium species. No effect of Filobasidium spp. was seen on epithelial wound healing in scratch assays. In conclusion, the enriched presence of Filobasidium spp. in donor feces is associated with the positive response to FMT for patients with UC and hence it may serve as a predictive fungal biomarker for successful FMT.
Keyphrases
- gene expression
- ulcerative colitis
- dna methylation
- end stage renal disease
- chronic kidney disease
- ejection fraction
- cell therapy
- newly diagnosed
- disease activity
- prognostic factors
- peritoneal dialysis
- clinical trial
- rheumatoid arthritis
- ultrasound guided
- stem cells
- mesenchymal stem cells
- open label
- microbial community
- patient reported
- double blind
- systemic lupus erythematosus
- amino acid
- peripheral blood
- platelet rich plasma
- human health