Turning up the heat on essential E. coli genes.

Published in: Molecular systems biology (2023)

Temperature-sensitive (TS) alleles create tunable thermoswitches to deplete essential cellular activities and are used to dissect gene function. In their recent study, Link and colleagues (Schramm et al 2023) use a CRISPR-based approach to systematically create TS alleles across essential genes in E. coli.

Keyphrases

genome wide
genome wide identification
escherichia coli
dna methylation
copy number
genome wide analysis
crispr cas
heat stress
energy transfer