The Effect of Rainbow trout (Onchorhynchus mykiss) collagen Incorporated with Exopolysaccharides Derived from Rhodotorula mucilaginosa sp. on burn healing.

Burns are one of physically the debilitating injuries could be potentially fatal, therefore, providing appropriate coverage in order to reduce possible mortality risk and accelerate wound healing is mandatory. In this study, collagen/exopolysaccharide (Col/EPS 1-3%) scaffolds were synthesized from Rainbow trout (Oncorhynchus mykiss) skin's incorporated with Rhodotorula mucilaginosa sp. GUMS16, respectively, for promoting Grade 3 burn wound healing. In this regard, physicochemical characterizations and consequently, biological properties of the Col/EPS scaffolds were tested. The results showed the presence of EPS did not affect the minimum porosity dimensions while raising the EPS amount significantly reduced the maximum porosity dimensions. TGA, FTIR spectroscopy, and tensile properties results confirmed the successful incorporation of the EPS into Col scaffolds. Furthermore, the biological results showed the increasing EPS did not affect Col biodegradability, cell viability and the use of Col/EPS 1% on the rat models displayed a faster healing rate. Finally, histopathological examination revealed the Col/EPS 1% treatment accelerated wound healing, through the greater re-epithelialization and dermal remodeling, more abundant fibroblast cells and Col accumulation. Our finding suggested that Col/EPS 1% promotes dermal wound healing via antioxidant and anti-inflammatory activities which it can be a potential medical process in the treatment of burn wounds. This article is protected by copyright. All rights reserved.