Human wharton-jelly mesenchymal stromal cells reversed apoptosis and prevented multi-organ damage in a newborn model of experimental asphyxia.
Bilge KocabiyikErkan GumusBurcin Irem AbasAyse AnikOzge CevikPublished in: Journal of obstetrics and gynaecology : the journal of the Institute of Obstetrics and Gynaecology (2023)
In this study, the effect of applying wharton jelly mesenchymal stromal cells (WJ-MSC) isolated from the human umbilical cord tissue on the neonatal mouse model caused experimental asphyxia in mice was investigated. WJ-MSC surface markers (CD44, CD90, CD105) were characterised by immunofluorescence staining, and pluripotency genes (Nanog, Oct-4, Sox-2) were characterised by qPCR. Blood, prefrontal cortex, cerebellum, hippocampus, lung, heart, kidney, and liver tissues were analysed twenty days after subcutaneously administered WJ-MSC. WJ-MSC administration significantly decreased serum TNF-α, NSE, GFAP, and IL-6 levels in the asphyxia mice. It was determined that WJ-MSC application in tissues accelerated cell regeneration and decreased oxidative stress. In conclusion, this study showed that multiorgan damage in asphyxia could be prevented by applying WJ-MSC at an early stage. Therefore, WJ-MSC application in infants with neonatal asphyxia in the clinic may be an innovative method in the future.
Keyphrases
- oxidative stress
- umbilical cord
- mesenchymal stem cells
- prefrontal cortex
- early stage
- endothelial cells
- mouse model
- stem cells
- gene expression
- bone marrow
- heart failure
- primary care
- cell death
- transcription factor
- dna damage
- ischemia reperfusion injury
- induced pluripotent stem cells
- cell proliferation
- single cell
- radiation therapy
- dna methylation
- genome wide
- mass spectrometry
- brain injury
- neoadjuvant chemotherapy
- cognitive impairment
- wild type
- heat stress
- atomic force microscopy
- rectal cancer
- high speed
- cell fate
- pi k akt