Cellular Effects of Cyclodextrins: Studies on HeLa Cells.
Ágnes RusznyákMercédesz PalicskóMilo MalangaEva FenyvesiLajos SzenteJudit VáradiIldikó BácskayMiklós VecsernyésKatalin Szászné Réti-NagyGábor VasváriÁdám HaimhofferFerenc FenyvesiPublished in: Molecules (Basel, Switzerland) (2022)
Cyclodextrins are high molecular weight, hydrophilic, cyclic, non-reducing oligosaccharides, applied as excipients for the improvement of the solubility and permeability of insoluble active pharmaceutical ingredients. On the other hand, beta-cyclodextrins are used as cholesterol sequestering agents in life sciences. Recently, we demonstrated the cellular internalization and intracellular effects of cyclodextrins on Caco-2 cells. In this study, we aimed to further investigate the endocytosis of (2-hydroxylpropyl)-beta-(HPBCD) and random methylated-beta-cyclodextrin (RAMEB) to test their cytotoxicity, NF-kappa B pathway induction, autophagy, and lysosome formation on HeLa cells. These derivatives were able to enter the cells; however, major differences were revealed in the inhibition of their endocytosis compared to Caco-2 cells. NF-kappa B p65 translocation was not detected in the cell nuclei after HPBCD or RAMEB pre-treatment and cyclodextrin treatment did not enhance the formation of autophagosomes. These cyclodextrin derivates were partially localized in lysosomes after internalization.
Keyphrases
- induced apoptosis
- cell cycle arrest
- signaling pathway
- cell death
- endoplasmic reticulum stress
- pi k akt
- oxidative stress
- single cell
- cell proliferation
- bone marrow
- combination therapy
- liquid chromatography
- reactive oxygen species
- fluorescent probe
- tandem mass spectrometry
- simultaneous determination
- structure activity relationship