Sequential extraction of the phosphate and collagen fractions of small bone samples for analysis of multiple isotope systems (δ18 OPO4 , δ13 C, δ15 N).

The new sequential extraction scheme combines the preparation for stable isotope analysis of bone mineral and organic phases, thus minimizing the amounts of sample needed and damage caused on a sample piece. The method may allow analysis of skeletal samples previously excluded from isotope analysis due to material limitations.