Iron restriction inside macrophages regulates pulmonary host defense against Rhizopus species.
Angeliki M AndrianakiIrene KyrmiziKalliopi ThanopoulouClara BaldinElias DrakosSameh S M SolimanAmol C ShettyCarrie McCrackenTonia AkoumianakiKostas StylianouPetros IoannouCharalampos PontikoglouHelen A PapadakiMaria TzardiValerie BelleEmilien EtienneAnne BeauvaisGeorge SamonisDimitrios P KontoyiannisEvangelos AndreakosVincent M BrunoAshraf S IbrahimGeorgios ChamilosPublished in: Nature communications (2018)
Mucormycosis is a life-threatening respiratory fungal infection predominantly caused by Rhizopus species. Mucormycosis has incompletely understood pathogenesis, particularly how abnormalities in iron metabolism compromise immune responses. Here we show how, as opposed to other filamentous fungi, Rhizopus spp. establish intracellular persistence inside alveolar macrophages (AMs). Mechanistically, lack of intracellular swelling of Rhizopus conidia results in surface retention of melanin, which induces phagosome maturation arrest through inhibition of LC3-associated phagocytosis. Intracellular inhibition of Rhizopus is an important effector mechanism, as infection of immunocompetent mice with swollen conidia, which evade phagocytosis, results in acute lethality. Concordantly, AM depletion markedly increases susceptibility to mucormycosis. Host and pathogen transcriptomics, iron supplementation studies, and genetic manipulation of iron assimilation of fungal pathways demonstrate that iron restriction inside macrophages regulates immunity against Rhizopus. Our findings shed light on the pathogenetic mechanisms of mucormycosis and reveal the role of macrophage-mediated nutritional immunity against filamentous fungi.
Keyphrases
- iron deficiency
- immune response
- single cell
- reactive oxygen species
- dendritic cells
- pulmonary hypertension
- liver failure
- respiratory failure
- skeletal muscle
- inflammatory response
- african american
- high fat diet induced
- type iii
- aortic dissection
- solid phase extraction
- high resolution mass spectrometry
- innate immune
- tandem mass spectrometry