A novel and simple heat-based method eliminates the highly detrimental effect of xylene deparaffinization on acid-fast stains.

Pedro F MarinhoSoraia L VieiraTânia G CarvalhoMaria C PeleteiroThomas Hanscheid

Published in: American journal of clinical pathology (2023)

Auramine O can be applied for nucleic acid staining of mycobacteria in tissues producing typical beaded patterns. Acid-fast staining depends heavily on the integrity of the mycobacterial cell wall, which xylene appears to damage. A solvent-free tissue deparaffinization method has the potential to increase mycobacterial detection significantly.

Keyphrases

nucleic acid
cell wall
mycobacterium tuberculosis
gene expression
flow cytometry
oxidative stress
ionic liquid
risk assessment
climate change
human health
solar cells