Automated glycan assembly of branched β-(1,3)-glucans to identify antibody epitopes.

β-(1,3)-Glucans exhibit immunomodulatory and anti-tumor effects. Since the isolation of pure β-(1,3)-glucan oligosaccharides from natural sources is complicated, especially when certain branching patterns are desired, chemical synthesis is frequently the only means of accessing these molecules. We report the iterative automated glycan assembly (AGA) of conjugation-ready linear and branched β-(1,3)-glucan oligosaccharides.