TGFβ1-induced SMAD2/3 and SMAD1/5 phosphorylation are both ALK5-kinase-dependent in primary chondrocytes and mediated by TAK1 kinase activity.

Arjan P M van CaamWojciech MadejAmaya Garcia de VinuesaMarie-José GoumansPeter Ten DijkeEsmeralda Blaney DavidsonPeter van der Kraan

Published in: Arthritis research & therapy (2017)

Our data suggest that ALK5 kinase activity plays a central role in both TGFβ-induced Smad1/5 and Smad2/3 phosphorylation, making it difficult to separate both pathways with the use of currently available small molecule inhibitors. Furthermore, our data regarding (5Z)-7-Oxozeaenol suggest that TAK1 facilitates Smad-dependent signaling.

Keyphrases

transforming growth factor
epithelial mesenchymal transition
protein kinase
small molecule
high glucose
diabetic rats
electronic health record
tyrosine kinase
drug induced
oxidative stress
deep learning
stress induced