A paper-based sensor for rapid and ultrasensitive detection of ibuprofen in water and herbal tea.
Shaozhen LiWenliang GeSteven SuryoprabowoJie LiuHua KuangJianping ZhuLiqiang LiuChuanlai XuPublished in: The Analyst (2021)
As the use of non-steroidal anti-inflammatory drugs (NSAIDS) increases, their side effects have also attracted attention. Ibuprofen is one of the most widely-used NSAIDs. In this study, we screened the highly-sensitive and specific antibody 6E10, with an IC50 of 1.92 ng mL-1, and a linear range of 0.53-6.97 ng mL-1. In this study, we developed a rapid lateral flow immunochromatographic assay (ICA) strip method to detect ibuprofen in water or herbal tea. The cut-off limit of the strip is 10 ng mL-1 in water, and concentrations as low as 1 ng mL-1 can be detected in herbal tea samples, with the results obtained by the naked eye within 6 min. All the data were confirmed by high performance liquid chromatography-quadrupole time of flight-mass spectrometry (HPLC-QTOF-MS). This lateral-flow ICA strip is thus a rapid tool for on-site detection and screening of ibuprofen in water and herbal tea.
Keyphrases
- anti inflammatory drugs
- high performance liquid chromatography
- loop mediated isothermal amplification
- mass spectrometry
- ms ms
- tandem mass spectrometry
- simultaneous determination
- label free
- solid phase extraction
- liquid chromatography
- gold nanoparticles
- high throughput
- molecularly imprinted
- postoperative pain
- quantum dots
- big data
- real time pcr
- electronic health record
- high resolution
- deep learning