Asymmetric inheritance of spindle microtubule-organizing centres preserves replicative lifespan.
Javier Manzano-LópezLaura MatellánAlejandra Álvarez-LlamasJosé Carlos Blanco-MiraFernando Monje-CasasPublished in: Nature cell biology (2019)
The differential distribution of the microtubule-organizing centres (MTOCs) that orchestrate spindle formation during cell division is a fascinating phenomenon originally described in Saccharomyces cerevisiae and later found to be conserved during stem cell divisions in organisms ranging from Drosophila to humans. Whether predetermined MTOC inheritance patterns fulfil any biological function is however unknown. Using a genetically designed S. cerevisiae strain that displays a constitutively inverted MTOC fate, we demonstrate that the asymmetric segregation of these structures is critical to ensure normal levels of the Sir2 sirtuin and correct localization of the mitochondrial inheritance regulator Mfb1, and therefore to properly distribute functional mitochondria and protein aggregates between the mother and daughter cells. Consequently, interfering with this process severely accelerates cellular ageing.
Keyphrases
- saccharomyces cerevisiae
- mitochondrial dna
- stem cells
- induced apoptosis
- transcription factor
- cell cycle arrest
- copy number
- oxidative stress
- cell therapy
- cell death
- single cell
- solid state
- high resolution
- signaling pathway
- endoplasmic reticulum stress
- gram negative
- binding protein
- mesenchymal stem cells
- endoplasmic reticulum
- protein protein
- amino acid
- mass spectrometry
- gene expression
- perovskite solar cells