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Pulsed Interleaved MINFLUX.

Luciano A MasulloFlorian SteinerJonas ZähringerLucía F LopezJohann BohlenLars RichterFiona ColePhilip TinnefeldFernando D Stefani
Published in: Nano letters (2020)
We introduce p-MINFLUX, a new implementation of the highly photon-efficient single-molecule localization method with a simplified experimental setup and additional fluorescence lifetime information. In contrast to the original MINFLUX implementation, p-MINFLUX uses interleaved laser pulses to deliver the doughnut-shaped excitation foci at a maximum repetition rate. Using both static and dynamic DNA origami model systems, we demonstrate the performance of p-MINFLUX for single-molecule localization nanoscopy and tracking, respectively. p-MINFLUX delivers 1-2 nm localization precision with 2000-1000 photon counts. In addition, p-MINFLUX gives access to the fluorescence lifetime enabling multiplexing and super-resolved lifetime imaging. p-MINFLUX should help to unlock the full potential of innovative single-molecule localization schemes.
Keyphrases
  • single molecule
  • living cells
  • atomic force microscopy
  • healthcare
  • primary care
  • magnetic resonance
  • quality improvement
  • high resolution
  • photodynamic therapy
  • climate change
  • fluorescent probe