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Visible light responsive photoacids for subcellular pH and temperature correlated fluorescence sensing.

Yu ChengXueqing MaJingying ZhaiXiaojiang Xie
Published in: Chemical communications (Cambridge, England) (2023)
Liao's photoacids (PAs) are a well-known type of visible light-responsive photoswitches. Here, taking advantage of the temperature-dependent thermal relaxation from the ring-closed to the ring-opened forms, PAs are proposed for the first time as a fluorescent temperature sensor in cells. The logarithmic lifetime (ln  τ ) of the ring-closed spiro-form exhibited an excellent linear response to the reciprocal of the temperature. In addition, the fluorescent ring-opened PAs were able to highlight lysosomes and responded to lysosomal pH changes. These properties made the PAs promising fluorescent probes in the sensing of subcellular pH and temperature.
Keyphrases
  • visible light
  • living cells
  • quantum dots
  • single molecule
  • label free
  • oxidative stress
  • cell proliferation
  • energy transfer