Panhematopoietic RNA barcoding enables kinetic measurements of nucleate and anucleate lineages and the activation of myeloid clones following acute platelet depletion.

Edyta E WojtowiczJayna J MistryVladimir UzunCharlotte HellmichAnita ScoonesDesmond W ChinLaura M KettyleFrancesca GrassoAllegra M LordDavid J WrightGraham J EtheringtonPetter S WollMirjam E BelderbosKristian M BowlesClaus NerlovWilfried HaertyLeonid V BystrykhSten Eirik W JacobsenStuart A RushworthIain C Macaulay

Published in: Genome biology (2023)

Our approach enables kinetic studies of multi-lineage output in the peripheral blood and transcriptional heterogeneity of individual hematopoietic stem cells. Our results give a unique insight into hematopoietic stem cell reactivation upon platelet depletion and of clonal dynamics in both steady state and under stress.

Keyphrases

hematopoietic stem cell
peripheral blood
stem cells
bone marrow
single cell
liver failure
respiratory failure
gene expression
dendritic cells
transcription factor
acute myeloid leukemia
drug induced
case control
intensive care unit
stress induced
nucleic acid