Simultaneous detection of lung fusions using a multiplex RT-PCR next generation sequencing-based approach: a multi-institutional research study.
Cecily P VaughnJosé Luís CostaHarriet E FeilotterRosella PetraroliVarun BagaiAnna Maria RachiglioFederica Zito MarinoBastiaan TopsHenriette M KurthKazuko SakaiAndrea MafficiniRoy R L BastienAnne ReimanDelphine Le CorreAlexander BoagSusan CrockerMichel BihlAstrid HirschmannAldo ScarpaJosé Carlos MachadoHélène BlonsOrla SheilsKelli BramlettMarjolijn J L LigtenbergIan A CreeNicola NormannoKazuto NishioPierre Laurent-PuigPublished in: BMC cancer (2018)
This methodology enables simultaneous detection of multiple ALK, ROS1, RET, and NTRK1 gene fusion transcripts in a single panel, enhanced by an integrated analysis solution. The assay performs well on limited amounts of input RNA (10 ng) and offers an integrated single assay solution for detection of actionable fusions in lung adenocarcinoma, with potential savings in both cost and turn-around-time compared to the combination of all four assays by other methods.