A mitochondria-targeted rhodol fluorescent probe for imaging of hydrogen peroxide in living cells.
Linlin LvWeiwei LuoQuanping DiaoPublished in: Analytical methods : advancing methods and applications (2022)
Hydrogen peroxide (H 2 O 2 ) is a main member of reactive oxygen species (ROS) in cells that has a significant impact on various physiological and pathological processes of organisms. Here, we designed and synthesized a new type of fluorescent probe Rhodol-OAc for the specific detection of H 2 O 2 . The probe had good water solubility, high selectivity and sensitivity to H 2 O 2 , low cytotoxicity, excellent mitochondrial targeting ability, etc. It was successfully applied in the imaging of exogenous and endogenous H 2 O 2 in living cells. In addition, theoretical calculations were carried out to clarify the luminescence mechanism of the probe. More importantly, we successfully applied the probe to indirectly detect xanthine and glucose, the metabolism of which generates H 2 O 2 , and achieved satisfactory results.
Keyphrases
- living cells
- hydrogen peroxide
- fluorescent probe
- reactive oxygen species
- nitric oxide
- high resolution
- cell death
- single molecule
- induced apoptosis
- cancer therapy
- cell cycle arrest
- oxidative stress
- dna damage
- molecular dynamics
- quantum dots
- molecular dynamics simulations
- type diabetes
- fluorescence imaging
- adipose tissue
- signaling pathway
- endoplasmic reticulum stress
- metabolic syndrome
- mass spectrometry
- gram negative
- insulin resistance
- photodynamic therapy
- glycemic control
- light emitting