Study on the Molecular Mechanism of the Herbal Couple Sparganii Rhizoma-Curcumae Rhizoma in the Treatment of Lung Cancer Based on Network Pharmacology.
Shuying DaiGaochenxi ZhangFangmin ZhaoQi-Jin ShuPublished in: Evidence-based complementary and alternative medicine : eCAM (2021)
A total of seven bioactive components were identified by network pharmacological analysis. Through enrichment analyses, it was found that the mechanism of action mainly involved mitochondrial-mediated caspase-dependent cell apoptosis signaling pathways. The results of molecular docking showed that the bioactive components in HCSC have a good affinity with the target proteins (ALB, BCL2L1, ESR1, HRAS, MAP2K1, MAPK14, and SIRT1). LC-MS confirmed that formononetin and bisdemethoxycurcumin were present in the HCSC freeze-dried powder, consistent with the prediction. The results of in vitro experiments on NCI-H1975 cells confirmed that HCSC can upregulate the mitochondrial-mediated caspase-dependent apoptosis signaling pathway by inducing the cleavage of caspase-3, caspase-9, and PARP, consistent with the network pharmacology prediction. Further, the qi deficiency and blood stasis associated with TCM syndrome can be treated with HCSC.
Keyphrases
- induced apoptosis
- oxidative stress
- signaling pathway
- molecular docking
- endoplasmic reticulum stress
- cell death
- pi k akt
- cell cycle arrest
- dna damage
- ischemia reperfusion injury
- epithelial mesenchymal transition
- molecular dynamics simulations
- cell proliferation
- dna repair
- combination therapy
- case report
- dna binding
- high density
- data analysis