A detached leaf assay for testing transient gene expression and gene editing in cowpea (Vigna unguiculata [L.] Walp.).

Martina JuranićDilrukshi S K NagahatennaRigel Salinas-GamboaMelanie L HandNidia Sánchez-LeónWeng Herng LeongTracy HowNatalia BazanovaAndrew SpriggsJean-Philippe Vielle-CalzadaAnna M G Koltunow

Published in: Plant methods (2020)

The transient, detached cowpea leaf assay, and supporting analytical methods developed, provide a rapid and reproducible means for testing gene expression constructs, and constructs for inducing mutagenesis in genes involved in both vegetative and reproductive developmental programs. The method and tested editing constructs and components have potential application for a range of crop legumes.

Keyphrases

gene expression
crispr cas
dna methylation
high throughput
cerebral ischemia
climate change
public health
risk assessment
quantum dots