Banhasasim-Tang Ameliorates Spatial Memory by Suppressing Oxidative Stress through Regulation of ERK/p38 Signaling in Hippocampus of Mice.
Malk Eun PakYou-Chang OhYeo Jin ParkJae Kwang KimMin-Gyeong ShinJong Hyuk YoonYoung-Hoon GoPublished in: Oxidative medicine and cellular longevity (2021)
Since ancient times, Banhasasim-tang (BHS) has been used to treat functional dyspepsia in East Asia. Here, we aimed to determine the protective action of BHS on hippocampal neurons against oxidative stress. We investigated the functional effect of BHS on a scopolamine-induced mouse model, and molecular analysis was performed in glutamate-induced HT22 cells. We observed that BHS administration ameliorated memory dysfunction in scopolamine-treated mice. BHS administration also increased neuronal survival and acetylcholine activity and phosphorylation of extracellular signal-regulated kinase (ERK) and cAMP response element-binding protein (CREB) in the hippocampus of mice. In hippocampal cells, BHS treatment rescued glutamate-induced cytotoxicity, apoptosis, and oxidative stress. We observed an increase of HO-1 and a decrease of Nrf2 protein expression in glutamate-induced oxidative stress; however, the expression level of these proteins was significantly rescued by BHS treatment. BHS treatment also regulated phosphorylation of p38, p53, ERK, and CREB. Therefore, our data indicated that BHS may reduce oxidative stress through regulation of ERK-CREB and p38-p53 signaling in the hippocampus, resulting in decreased neuronal damage and improved memory in rodent models of neurodegenerative disease.
Keyphrases
- oxidative stress
- diabetic rats
- induced apoptosis
- signaling pathway
- pi k akt
- cell cycle arrest
- binding protein
- cerebral ischemia
- mouse model
- dna damage
- ischemia reperfusion injury
- high glucose
- cell proliferation
- endoplasmic reticulum stress
- working memory
- transcription factor
- cell death
- type diabetes
- machine learning
- protein kinase
- drug induced
- tyrosine kinase
- metabolic syndrome
- spinal cord
- replacement therapy
- skeletal muscle
- adipose tissue
- electronic health record
- irritable bowel syndrome