Acoustofluidics-Assisted Fluorescence-SERS Bimodal Biosensors.
Nanjing HaoZhichao PeiPengzhan LiuHunter BachmanTy Downing NaquinPeiran ZhangJinxin ZhangLiang ShenShujie YangKaichun YangShuaiguo ZhaoTony Jun HuangPublished in: Small (Weinheim an der Bergstrasse, Germany) (2020)
Acoustofluidics, the fusion of acoustics and microfluidic techniques, has recently seen increased research attention across multiple disciplines due in part to its capabilities in contactless, biocompatible, and precise manipulation of micro-/nano-objects. Herein, a bimodal signal amplification platform which relies on acoustofluidics-induced enrichment of nanoparticles is introduced. The dual-function biosensor can perform sensitive immunofluorescent or surface-enhanced Raman spectroscopy (SERS) detection. The platform functions by using surface acoustic waves to concentrate nanoparticles at either the center or perimeter of a glass capillary; the concentration location is adjusted simply by varying the input frequency. The immunofluorescence assay is achieved by concentrating fluorescent analytes and functionalized nanoparticles at the center of the microchannel, thereby improving the visibility of the fluorescent output. By modifying the inner wall of the glass capillary with plasmonic Ag nanoparticle-deposited ZnO nanorod arrays and focusing analytes toward the perimeter of the microchannel, SERS sensing using the same device setup is achieved. Nanosized exosomes are used as a proof-of-concept to validate the performance of the acoustofluidic bimodal biosensor. With its sample-enrichment functionality, bimodal sensing, short processing time, and minute sample consumption, the acoustofluidic chip holds great potential for the development of lab-on-a-chip based analysis systems in many real-world applications.
Keyphrases
- label free
- high throughput
- raman spectroscopy
- quantum dots
- sensitive detection
- energy transfer
- circulating tumor cells
- gold nanoparticles
- stem cells
- single cell
- working memory
- loop mediated isothermal amplification
- high glucose
- walled carbon nanotubes
- room temperature
- endothelial cells
- capillary electrophoresis
- drug induced
- ionic liquid
- high resolution
- risk assessment
- data analysis
- iron oxide