Osteogenic Differentiation Modulates the Cytokine, Chemokine, and Growth Factor Profile of ASCs and SHED.
Federico Davide MussanoTullio GenovaSara PetrilloIlaria RoatoRiccardo FerraciniLuca MunaronPublished in: International journal of molecular sciences (2018)
Great efforts have been made to improve bone regeneration techniques owing to a growing variety of sources of stem cells suitable for autologous transplants. Specifically, adipose-derived stem cells (ASCs) and stems cells from human exfoliated deciduous teeth (SHED) hold great potential for bone tissue engineering and cell therapy. After a preliminary characterization of the main biomolecules ASCs and SHED released in their conditioned media, cells were kept both in normal and osteo-inducing conditions. Conventional assays were performed to prove their osteogenic potential such as quantitative real-time polymerase chain reaction (qRT-PCR) (for RUNX-2, collagen type I, osteopontin and osteonectin), alkaline phosphatase activity, osteocalcin production, and von Kossa staining. Conditioned media were tested again after the osteogenic induction and compared to maintaining condition both at base line and after 14 days of culture. The osteogenic condition inhibited the release of all the biomolecules, with the exception, concerning SHED, of growth-regulated alpha protein precursor (GROα), and, to a lesser extent, interleukin (IL)-8. In conclusion, our data support that undifferentiated ASCs and SHED may be preferable to committed ones for general cell therapy approaches, due to their higher paracrine activity. Osteoinduction significantly affects the cytokine, chemokine, and growth factor profile in a differential way, as SHED kept a more pronounced pro-angiogenic signature than ASCs.
Keyphrases
- cell therapy
- growth factor
- mesenchymal stem cells
- tissue engineering
- stem cells
- bone regeneration
- bone marrow
- induced apoptosis
- transcription factor
- endothelial cells
- cell cycle arrest
- high throughput
- electronic health record
- oxidative stress
- drinking water
- induced pluripotent stem cells
- cell death
- human health
- big data
- wound healing
- risk assessment
- signaling pathway
- machine learning
- endoplasmic reticulum stress
- climate change
- single cell
- flow cytometry
- soft tissue