Login / Signup

A feasibility study of zebrafish embryo irradiation with laser-accelerated protons.

Thomas F RöschZoltán SzabóDaniel HaffaJianhui BinSzilvia BrunnerFranz Siegfried EnglbrechtAnna A FriedlYing GaoJens HartmannPeter HilzChristian KreuzerFlorian H LindnerTobias M OstermayrRóbert PolanekMartin SpeicherEmília R SzabóDerya TarayTünde TőkésMatthias WuerlKatia ParodiKatalin HideghétyJoerg Schreiber
Published in: The Review of scientific instruments (2021)
The development from single shot basic laser plasma interaction research toward experiments in which repetition rated laser-driven ion sources can be applied requires technological improvements. For example, in the case of radio-biological experiments, irradiation duration and reproducible controlled conditions are important for performing studies with a large number of samples. We present important technological advancements of recent years at the ATLAS 300 laser in Garching near Munich since our last radiation biology experiment. Improvements range from target positioning over proton transport and diagnostics to specimen handling. Exemplarily, we show the current capabilities by performing an application oriented experiment employing the zebrafish embryo model as a living vertebrate organism for laser-driven proton irradiation. The size, intensity, and energy of the laser-driven proton bunches resulted in evaluable partial body changes in the small (<1 mm) embryos, confirming the feasibility of the experimental system. The outcomes of this first study show both the appropriateness of the current capabilities and the required improvements of our laser-driven proton source for in vivo biological experiments, in particular the need for accurate, spatially resolved single bunch dosimetry and image guidance.
Keyphrases
  • high speed
  • deep learning
  • single cell
  • radiation induced
  • radiation therapy
  • metabolic syndrome
  • adipose tissue
  • machine learning
  • skeletal muscle
  • high resolution