Design and Application of a DNA-Encoded Macrocyclic Peptide Library.
Zhengrong ZhuAlex ShaginianLaShadric C GradyThomas O'KeeffeXiangguo E ShiChristopher P DavieGraham L SimpsonJeffrey A MesserGhotas EvindarRobert N BreamPraew P ThansandoteNaomi R PrenticeAndrew M MasonSandeep PalPublished in: ACS chemical biology (2017)
A DNA-encoded macrocyclic peptide library was designed and synthesized with 2.4 × 1012 members composed of 4-20 natural and non-natural amino acids. Affinity-based selection was performed against two therapeutic targets, VHL and RSV N protein. On the basis of selection data, some peptides were selected for resynthesis without a DNA tag, and their activity was confirmed.