DETERMINATION OF VENLAFAXINE, VILAZODONE AND THEIR MAIN ACTIVE METABOLITES IN HUMAN SERUM BY HPLC-DAD AND HPLC-MS.
Anna PetruczynikKarol WroblewskiMalgorzata Szultka-MlynskaBoguslaw BuszewskHanna Karakula-JuchnowiczJacek GajewskiJustyna Morylowska-TopolskaMonika Waksmundzka-HajnosiPublished in: Acta poloniae pharmaceutica (2018)
A high performance liquid chromatography (HPLC) method for simultaneous analysis of venlafaxine and its major metabolite 0-desmethylvenlafaxine and vilazodone and its methabolite M10 have been devel- oped and validated. Chromatography was performed on the Phenyl-Hexyl column with mobile phase containing methanol, acetate buffer at pH 3.5 and diethylamine. The application of stationary phase with 7r-7c moieties and mobile phase containing diethylamine as silanol blocker lets to obtain double protection against silanols and thus very high theoretical plate numbers were obtained. The good separation selectivity, good peaks' symmetry and very high systems efficiency for all investigated compounds were obtained in applied chromatographic system. The method is very efficient and suitable for the analysis of investigated drugs and their metabolites in human serum for patients' pharmacotherapy control.
Keyphrases
- high performance liquid chromatography
- ms ms
- simultaneous determination
- liquid chromatography
- solid phase extraction
- tandem mass spectrometry
- mass spectrometry
- molecularly imprinted
- end stage renal disease
- ejection fraction
- chronic kidney disease
- newly diagnosed
- atomic force microscopy
- multiple sclerosis
- peritoneal dialysis
- high resolution
- prognostic factors
- smoking cessation
- angiotensin ii
- angiotensin converting enzyme