Self-assembled NIR-II Fluorophores with Ultralong Blood Circulation for Cancer Imaging and Image-guided Surgery.
Yang LiJianfeng GaoShuping WangMingxia DuXiaowen HouTian TianXue QiaoZhi-Quan TianPeter J StangShijun LiXuechuan HongYuling XiaoPublished in: Journal of medicinal chemistry (2021)
Complete excision of the last remaining 1-2% of tumor tissue without collateral damage remains particularly challenging. Herein, we report thiophenthiadiazole (TTD)-derived fluorophores L6-PEG nk ( n = 1, 2, 5) as new-generation NIR-II (1000-1700 nm) probes with exceptional nonfouling performance and significantly high fluorescence quantum yields in water. L6-PEG 2k can self-assemble into vesicular micelles and exhibited minimal immunogenicity, low binding affinities, ultralong blood circulation ( t 1/2 = 59.5 h), and a supercontrast ratio in vivo . Most importantly, L6-PEG 2k achieved excellent in vivo CT-26 and U87MG tumor targeting and accumulation (>20 d) through intraperitoneal or intravenous injection. A subcutaneous U87MG tumor and orthotopic brain glioma were successfully resected under NIR-II FIGS in our animal model via intraperitoneal injection in an extended time window (48-144 h). This study highlights the potential of using L6-PEG 2K as self-assembling molecular probes with long-circulation persistence for routine preoperative tumor assessment and precise intraoperative image-guided resection.
Keyphrases
- functional connectivity
- drug delivery
- fluorescence imaging
- photodynamic therapy
- drug release
- single molecule
- minimally invasive
- small molecule
- cancer therapy
- high resolution
- oxidative stress
- patients undergoing
- white matter
- living cells
- lymph node
- multiple sclerosis
- coronary artery disease
- squamous cell carcinoma
- low dose
- molecular dynamics
- ultrasound guided
- risk assessment
- transcription factor
- brain injury
- blood brain barrier
- surgical site infection
- quantum dots