In this study, the chemical components of ethanol extract from the aromatic parts of Syringa oblata were systematically separated and purified by silica gel column chromatography, thin layer plate preparation and liquid phase preparation. Combined with ultraviolet analyzer(UV), infrared analyzer(IR), nuclear magnetic resonance analyzer(NMR), high resolution mass spectrometer(HR-ESI-MS), X-ray diffraction and other spectrum technology as well as literature physicochemical data comparison methods for structural identification, a total of 10 compounds were identified. They were identified as oblatanoid D(1),(-)-T-muurolol(2), oblatanoid E-G(3-5), 14-noreudesma-3-hydroxy-3-en-2,9-dione(6), 1-isopropyl-2,7-dimethylnaphthalene(7), isocoradiol(8), α-calacorene(9), cadin-4-en-1-β-ol(10). Compound 1 is a new sesquiterpene compound that has not been reported, and the other 9 compounds are isolated from S. oblata for the first time. The compound 1 has a significant protective effect on the LPS-induced inflammatory injury model of RAW264.7 cells.
Keyphrases
- high resolution
- mass spectrometry
- lps induced
- magnetic resonance
- liquid chromatography
- ms ms
- inflammatory response
- high speed
- tandem mass spectrometry
- induced apoptosis
- oxidative stress
- molecularly imprinted
- systematic review
- multiple sclerosis
- high performance liquid chromatography
- cell cycle arrest
- solid phase extraction
- ionic liquid
- magnetic resonance imaging
- big data
- electronic health record
- anti inflammatory
- cell death
- computed tomography
- electron microscopy
- amino acid