Simultaneous determination of boscalid and fludioxonil in grape and soil under field conditions by gas chromatography/tandem triple quadrupole mass spectrometry.
Haizhen ZhangA'wei ZhangMin HuangWeiwei YuZhurui LiSizhuo WuKunming ZhengKankan ZhangDeyu HuPublished in: Biomedical chromatography : BMC (2017)
A gas chromatography-tandem mass spectrometry method was developed and validated to simultaneously determine boscalid and fludioxonil in grape and soil samples. These samples were extracted with 10 mL of acetonitrile and purified using a mixed primary secondary amine/octadecylsilane sorbent. The method showed good linearity (R2 > 0.99) in the calibration range 0.005-2 μg/mL for both pesticides. The limits of detection and quantification for the two analytes in grape and soil were 0.006 and 0.02 mg/kg, respectively. Fungicide recoveries in grape and soil were 81.18-92.11% for boscalid and 82.73-97.67% for fludioxonil with relative standard deviations of 1.31-10.31%. The established method was successfully applied to the residual analysis of boscalid and fludioxonil in real grape and soil samples. The terminal residue concentrations of boscalid and fludioxonil in grape samples collected from Anhui and Guizhou were <5 mg/kg (the maximum residue limit set by China) 7 days after the last application and 1 mg/kg (the maximum residue limit set by USA) 14 days after the last application. These results could provide guidance for the proper and safe use of boscalid and fludioxonil in grape and help the Chinese government to establish an MRL for fludioxonil in grape.
Keyphrases
- gas chromatography
- tandem mass spectrometry
- ultra high performance liquid chromatography
- simultaneous determination
- mass spectrometry
- liquid chromatography
- high performance liquid chromatography
- high resolution mass spectrometry
- solid phase extraction
- gas chromatography mass spectrometry
- liquid chromatography tandem mass spectrometry
- high resolution
- molecularly imprinted
- capillary electrophoresis
- amino acid
- plant growth
- risk assessment
- quantum dots