Early diagnostic imaging of pneumonia with an ultra-sensitive two-photon near-infrared fluorescent probe.
Erwang SongYoubo LaiHuixu LuYonghe TangLintao ZengWeiying LinPublished in: Journal of materials chemistry. B (2022)
The mortality rate of pneumonia increases significantly with the prolongation of illness. In the pursuit of a tool to accurately diagnose pneumonia in its early stages, we designed and synthesized a two-photon near-infrared fluorescent probe (DCQN) to identify increased concentrations of the inflammation marker SO 2 . The probe was found to specifically react with SO 2 by undergoing a 1,4-addition reaction to generate near-infrared fluorescence with good sensitivity (6 s), a large Stokes shift (110 nm) and low detection limit (1.49 nM). DCQN has near-infrared emission as well as good two-photon performance, which can image exogenous and endogenous SO 2 in cells and avoid interference from background fluorescence from cells. Furthermore, this study achieved accurate imaging of a pneumonia lesion site in deep tissues to provide a tool for the fluorescence diagnostic imaging of pneumonia in situ .
Keyphrases
- living cells
- fluorescent probe
- single molecule
- high resolution
- induced apoptosis
- cell cycle arrest
- respiratory failure
- community acquired pneumonia
- oxidative stress
- photodynamic therapy
- gene expression
- cardiovascular events
- energy transfer
- machine learning
- intensive care unit
- quantum dots
- extracorporeal membrane oxygenation
- mechanical ventilation