Multiplexed whole-animal imaging with reversibly switchable optoacoustic proteins.
Kanuj MishraMariia StankevychJuan Pablo Fuenzalida-WernerSimon GrassmannVipul GujratiYuanhui HuangUwe KlemmVeit R BuchholzVasilis NtziachristosAndre C StielPublished in: Science advances (2020)
We introduce two photochromic proteins for cell-specific in vivo optoacoustic (OA) imaging with signal unmixing in the temporal domain. We show highly sensitive, multiplexed visualization of T lymphocytes, bacteria, and tumors in the mouse body and brain. We developed machine learning-based software for commercial imaging systems for temporal unmixed OA imaging, enabling its routine use in life sciences.