Neuronal loss and gliosis in the rat striatum subjected to 15 and 30 minutes of middle cerebral artery occlusion.
Joon Ha ParkJeong Hwi ChoJi Hyeon AhnSoo Young ChoiTae-Kyeong LeeJae-Chul LeeBich Na ShinSeongkweon HongYong Hwan JeonYoung-Myeong KimIn Koo HwangYoung Joo LeeMoo-Ho WonIl Jun KangPublished in: Metabolic brain disease (2018)
Selective neuronal death or loss in certain brain regions has been well characterized in animal models of transient global cerebral ischemia. However, selective neuronal death in transient focal cerebral ischemia needs more investigation. Therefore, in this study, we studied selective neuronal death in the striatum (caudate putamen) of rats subjected to 15 or 30 min middle cerebral artery occlusion (MCAO). Neuronal death occurred in the dorsolateral field, not in the medial field in 30 min, not 15 min, MCAO-operated rats 5 days after MCAO using neuronal nuclear antigen immunohistochemistry and Fluoro-Jade B histofluorescence staining. In this group, immunoreactivity of glial fibrillary acidic protein in astrocytes was hardly shown in the dorsolateral field, although the immunoreactivity increased in the medial field. In addition, immunoreactivity of ionized calcium binding adapter molecule 1 in microglia was dramatically increased in the dorsolateral, not in the medial, field only in 30 min MCAO-operated rats. Briefly, these results show that at least 30 min of MCAO can evoke selective neuronal death, astrocytic dysfunction and microglial activation in the dorsolateral field of the rat striatum and suggest that a rat model of 30 min MCAO can be used to investigate mechanisms of neuronal death and gliosis following brief transient focal cerebral ischemic events for acute transient ischemic attack.
Keyphrases
- cerebral ischemia
- subarachnoid hemorrhage
- blood brain barrier
- brain injury
- prefrontal cortex
- middle cerebral artery
- working memory
- transcranial magnetic stimulation
- oxidative stress
- transcranial direct current stimulation
- neuropathic pain
- inflammatory response
- multiple sclerosis
- spinal cord injury
- spinal cord
- liver failure
- hepatitis b virus
- positron emission tomography
- pet ct
- transcription factor
- acute respiratory distress syndrome
- small molecule
- lipopolysaccharide induced