Simple Fluorescence Turn-On Chemosensor for Selective Detection of Ba2+ Ion and Its Live Cell Imaging.
Palanisamy RavichandiranSivakumar Allur SubramaniyanAntony Paulraj BellaPrincy Merlin JohnsonAe Rhan KimKwan Seob ShimChong Sam NaPublished in: Analytical chemistry (2019)
A phenoxazine-based fluorescence chemosensor 4PB [(4-(tert-butyl)-N-(4-((4-((5-oxo-5H-benzo[a]phenoxazin-6-yl)amino)phenyl)sulfonyl)phenyl)benzamide)] was designed and synthesized by a simple synthetic methods. The 4PB fluorescence chemosensor selectively detects Ba2+ in the existence of other alkaline metal ions. In addition, 4PB showed high selectivity and sensitivity for Ba2+ detection. The detection limit of 4PB was 0.282 μM and the binding constant was 1.0 × 106 M-1 in CH3CN/H2O (97.5:2.5 v/v, HEPES = 1.25 mM, pH 7.3) medium. This chemosensor functioned through the intramolecular charge transfer (ICT) mechanism, which was further confirmed by DFT studies. Live cell imaging in MCF-7 cells confirmed the cell permeability of 4PB and its capability for specific detection of Ba2+ in living cells.
Keyphrases
- aqueous solution
- heavy metals
- living cells
- single molecule
- loop mediated isothermal amplification
- fluorescent probe
- real time pcr
- high resolution
- label free
- energy transfer
- squamous cell carcinoma
- induced apoptosis
- single cell
- breast cancer cells
- signaling pathway
- lymph node metastasis
- quantum dots
- binding protein
- transcription factor
- room temperature
- dna binding
- photodynamic therapy