Zn-doped Mono- and Biphasic Calcium Phosphate Materials Derived from Agriculture Waste and Their Potential Biomedical Applications: Part I.

In this study, calcium phosphate materials were obtained via a simple, eco-friendly wet synthesis method using hen eggshells as a calcium source. It was shown that Zn ions were successfully incorporated into hydroxyapatite (HA). The obtained ceramic composition depends on the zinc content. When doped with 10 mol % of Zn, in addition to HA and Zn-doped HA, DCPD (dicalcium phosphate dihydrate) appeared and its content increased with the increase in Zn concentration. All doped HA materials exhibited antimicrobial activity against S. aureus and E. coli . Nevertheless, fabricated samples significantly decreased preosteoblast (MC3T3-E1 Subclone 4) viability in vitro, exerting a cytotoxic effect which probably resulted from their high ionic reactivity.