Establishment of porcine embryonic stem cells in simplified serum free media and feeder free expansion.

Hyerin ChoiDongjin OhMirae KimAli JawadHaomiao ZhengLian CaiJoohyeong LeeEunhye KimGabsang LeeHyewon JangChangjong MoonSang-Hwan Hyun

Published in: Stem cell research & therapy (2024)

These results indicated that inhibition of WNT and SRC was sufficient to establish pESCs capable of single-cell passaging and feeder-free expansion under serum-free conditions. The easy maintenance of pESCs facilitates their application in gene editing technology for agriculture and biomedicine, as well as lineage commitment studies.

Keyphrases

single cell
embryonic stem cells
climate change
cell proliferation
rna seq
high throughput
cell fate