Diagnostics and analysis of SARS-CoV-2: current status, recent advances, challenges and perspectives.
Tao DongMingyang WangJunchong LiuPengxin MaShuang PangWanjian LiuAihua LiuPublished in: Chemical science (2023)
The disastrous spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has induced severe public healthcare issues and weakened the global economy significantly. Although SARS-CoV-2 infection is not as fatal as the initial outbreak, many infected victims suffer from long COVID. Therefore, rapid and large-scale testing is critical in managing patients and alleviating its transmission. Herein, we review the recent advances in techniques to detect SARS-CoV-2. The sensing principles are detailed together with their application domains and analytical performances. In addition, the advantages and limits of each method are discussed and analyzed. Besides molecular diagnostics and antigen and antibody tests, we also review neutralizing antibodies and emerging SARS-CoV-2 variants. Further, the characteristics of the mutational locations in the different variants with epidemiological features are summarized. Finally, the challenges and possible strategies are prospected to develop new assays to meet different diagnostic needs. Thus, this comprehensive and systematic review of SARS-CoV-2 detection technologies may provide insightful guidance and direction for developing tools for the diagnosis and analysis of SARS-CoV-2 to support public healthcare and effective long-term pandemic management and control.
Keyphrases
- sars cov
- respiratory syndrome coronavirus
- healthcare
- systematic review
- coronavirus disease
- current status
- end stage renal disease
- mental health
- copy number
- chronic kidney disease
- loop mediated isothermal amplification
- ejection fraction
- early onset
- emergency department
- newly diagnosed
- prognostic factors
- peritoneal dialysis
- single molecule
- social media
- patient reported outcomes
- adverse drug
- sensitive detection
- single cell